Other

Part:BBa_I716333:Design

Designed by: nhu nguyen   Group: iGEM07_Berkeley_UC   (2007-07-11)

ThpC


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 6
    Illegal XhoI site found at 411
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 132
    Illegal BsaI.rc site found at 156
    Illegal BsaI.rc site found at 231


Design Notes


This part is built in Biobrick version 2.0. In this format, parts are flanked by BglII and BamHI restriction sites.


Source

ThpC
From IGEM07
PCR ThpC F/R on ThpC (421bp, BglII/XhoI)
Sub into pBca9145-Bca1144 (BgIII/XhoI, 2054+910 L)
Product is pBca9145-BBa I716333



ThpCF Forward BglII site anneals to ThpC ctagtAGATCTgtgatcgtccgatcgttcag

ThpAR Reverse XhoI site anneals to ThpC gtctactcgagtcaggcctcctcggtcagca


References